As was the case last week, the match will take place at Morecambe's Globe Arena - kick off 2:00pm.
A young City XI - featuring mainly apprentices and first year pros - drew 1-1 with Morecambe in the league fixture last Tuesday. Reece Webb-Foster netted City's goal with a superb curling effort from outside the box.
This time around, Phil Parkinson may look to use the opportunity to field some of his more senior players at Morecambe.
A number of players who didn't start the Sheffield United fixture for City in Sky Bet League 1 on Sunday could be involved in the cup tie at the Globe Arena.