Darby was part of a City eleven last weekend that grabbed the attention of a nation by stunning Premier League leaders Chelsea with an amazing come-from-behind victory over Jose Mourinho's stars at Stamford Bridge in the F.A Cup.
The win propelled Phil Parkinson's men into the spotlight and a place in the fifth round of the world's most famous domestic cup competition for the first time since 1997, but Darby insists now is the time to move on from the superb triumph.
With a sizeable crowd expected inside Valley Parade for Saturday's Colchester game following the Chelsea victory, Darby says City are keen to reward their followers with a win on their return to league action.
The City skipper said: "The Chelsea game is one that no one will forget in a hurry and we all enjoyed the weekend but it is important now we switch focus back onto the league.
"That is our bread and butter and ultimately what we are all judged on.
"From now until the end of the season, we still have a lot to achieve in the league.
"It is important that we get a good couple of days training under our belts now. I feel the lads have already switched back their focus onto the league and the Saturday's game against Colchester.
"It is a game that everyone is looking forward to.
"We've had a couple of recent defeats in the league and the boys are keen to get back to winning ways and get some points back on the board.
"It is always great to play at home. The supporters really get behind us from the first minute to the last. They always play their part and they are one of the main reasons why everyone wants to be involved in this club.
"To get a home fixture straight after this (Chelsea) win, I'm sure the place will be bouncing.
"We want to put a performance on for them and hopefully get the win.
"We're looking forward to the game and I'm sure they'll be a big crowd."
In other news, despite limping off towards the end of the Chelsea win, Filipe Morais should be fine for the Colchester fixture.
Morais is believed to have suffered only cramp and not a hamstring problem as first feared.
Meanwhile, a reserve side including Mark Yeates, Matt Urwin, Francois Zoko, Jason Kennedy, Matthew Dolan and Alan Sheehan lost 3-0 to a strong Burnley Development Squad XI at Carrington, Manchester this Tuesday.