After drawing with Rotherham 1-1 in the first leg, Preston fell to a 3-1 defeat on the night at the New York Stadium, losing 4-2 on aggregate, to ensure they will stay in the League 1 for another season.
Rotherham now move onto Wembley Stadium for the grand final to play Leyton Orient for a place in the Championship for the 2014/2015 campaign.
Preston's loss in the Play-Offs means Phil Parkinson's men now know twenty-one of the teams (please see below) who will help to make up their fixture schedule for next season.
The only places left to fill will be taken up by the losing League 1 Play-Off finalist and the winner of the League 2 Play-Offs.
BARNSLEY
BRISTOL CITY
CHESTERFIELD
COLCHESTER UNITED
COVENTRY CITY
CRAWLEY TOWN
CREWE ALEXANDRA
DONCASTER ROVERS
GILLINGHAM
MILTON KEYNES DONS
NOTTS COUNTY
OLDHAM ATHLETIC
BRISTOL CITY
CHESTERFIELD
COLCHESTER UNITED
COVENTRY CITY
CRAWLEY TOWN
CREWE ALEXANDRA
DONCASTER ROVERS
GILLINGHAM
MILTON KEYNES DONS
NOTTS COUNTY
OLDHAM ATHLETIC
PETERBOROUGH UNITED
PORT VALE
PORT VALE
PRESTON NORTH END
ROCHDALE
SCUNTHORPE UNITED
SHEFFIELD UNITED
SWINDON TOWN
WALSALL
YEOVIL TOWN
ROCHDALE
SCUNTHORPE UNITED
SHEFFIELD UNITED
SWINDON TOWN
WALSALL
YEOVIL TOWN